This is supplementary material for M. G. Pedersen and A. Sherman. 2009. Newcomer insulin secretory granules as a highly calcium-sensitive pool. PNAS 106(18):7432-6. PubMed
Fast single-cell protocol, corresponding to Fig. 2:
Slow islet protocol, corresponding to Fig. 3:
Period of “burst” is 30 sec; change parameter per to 360 for 6 minute bursts.